Knockdown of mammalian target of rapamycin complex 1 (mTORC1) by 35% or 60% during adulthood does not impact longevity or body size. (a) Gene diagrams depicting the inducible Cre recombinase and recombination of the Raptor allele after Cre recombinase expression. (b) Tissue expression of Cre recombinase as measured by β-galactosidase (β-gal) activity in the β-gal reporter strain. (c) Cre expression in brain regions measured as β-gal activity. (d) Cre expression in whole brain lysates measured as β-gal activity during development. (e) Raptor mRNA expression levels of in the brain of neuronal mTORC1 animals. (f) mTORC1 formation levels as determined by immunoprecipitation of mTOR and immunoblotting for Raptor and mTOR.(g) Representative images and (h) quantification of mTORC1 signaling, measured as phosphorylated rpS6 by immunofluorescence in hippocampal tissue sections. mTORC1 signaling levels as determined by (i) phosphorylated rpS6 and  (j) phosphorylated 4EBP in cortical lysates. (k) Cross-sectional body weight of adult male neuronal mTORC1 mice. (m) Lifespan of adult neuronal mTORC1 mice. Data are mean ± SEM. *p<0.05, ***p<0.001.